Analysis Note

Positive ControlCOS-7 cells

Application

Immunoblotting (1:200-1:1000) r>Immunofluorescence (1:250) r>Immunoprecipitation (1:20)r>

General description

This Anti-CHIP Rabbit pAb is validated for use in Immunoblotting for the detection of CHIP.

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~35 kDa CHIP protein.

Recognizes the ~35 kDa CHIP protein. May also detect bands at ~43, ~51, and ~70 kDa corresponding to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively.

Immunogen

a recombinant protein consisting of full-length human CHIP fused to a His Tag™ sequence

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Xu, W., et al. 2002. Proc. Natl. Acad. Sci. USA99, 12847.Demand, J., et al. 2001. Curr. Biol.11, 1569.Connell, P., et al. 2001. Nat. Cell Biol.3, 93. Jiang. J., et al. 2001. J. Biol. Chem. 276, 42938. Meacham, G.C., et al. 2001. Nat. Cell. Biol.3, 100.Ballinger, C.A., et al. 1999. Mol. Cell. Biol.19, 4535.

May also detect bands at 43, 51, and 70 kDa, wich correspond to the mono-ubiquitinated, di-ubiquitinated, and dimeric forms of CHIP, respectively. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)